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KMID : 0352720100340020113
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Journal of Ginseng Research
2010 Volume.34 No. 2 p.113 ~ p.121
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Physicochemical Characterization and NMR Assignments of Ginsenosides Rb₁, Rb₂, Rc, and Rd Isolated from Panax ginseng
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Cho Jin-Gyeong
Lee Min-Kyung
Lee Jae-Woong
Park Hee-Jung
Lee Dae-Young
Lee Youn-Hyung
Yang Deok-Chun
Baek Nam-In
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Abstract
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The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides Rb₁, Rb₂, Rc, and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published ¹H- and ¹³C-nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including ¹H?¹H correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.
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KEYWORD
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Diol ginsenoside, Fast atom bombardment/mass spectrometry, High-performance liquid chromatography, Nuclear magnetic resonance
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